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BioProject ID : KAP210017

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Project title : Genome analysis of Fisheries organisms

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Submitter

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Name Park Chan-Il
Organization Gyeongsang National University
Department Department of Seafood and Aquaculture Science

Date

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Registration Date 2021-02-03

Project Design

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NTIS Number 1525008270
Project Title Genome analysis of Fisheries organisms
Relevance environmental
Description "Project goal ○ Whole genome and transcriptome analyses of the starry flounder (Platichthys stellatus) - Genome sequencing of individual marine animals using the PacBio-Sequel and the HiSeq Paired-end methods - Transcriptome sequencing using the HiSeq Paired-end and the IsoSeq-Sequel sequencing methods, and DEG analysis using templates prepared from individual marine animals at varying time points after artificial infection or stimulation ○ Acquisition and analysis of useful genes - Selection of 15 or more useful genes from each marine animal - Expression analysis of 8 or more genes from the selected useful gene pool - Production of three or more of the selected useful gene products by recombinant expression systems or peptide synthesis and ligation to identify their functions "Project contents ○ Production of whole-genome data from the starry flounder - Genomic DNA extraction from a starry flounder population - Approximately 49 Gb of data production using the PacBio-Sequel (>20 kb) sequencing - Approximately 28 Gb of data production using the HiSeq Paired-end (2 × 101 bp) sequencing ○ Production of the starry flounder transcriptome data - 36 samples isolated and prepared at different time periods after artificial infection or stimulation for differential gene expression analysis - Production of 5 Gb of data per sample using the HiSeq Paired-end (2 × 101 bp) sequencing - Production of 5 Gb of data per cell (1 SMRT cell) using the IsoSeq-Sequel sequencing "Expected efficacy and applications ○ Providing technology to aid academic and industrial use of major aquaculture and aquatic biological resources through the completion of aquatic standard dielectric and establishment of a specialized genomic data platform for aquatic organisms ○ Accelerating research on aquacultured species by establishing economical methods to identify useful genes and gene markers ○ Creation of new industries by developing bio materials using the genomic big data "Expectation effectiveness and application ○ Providing technology for academic and industrial use of major aquaculture and aquatic biological resources through completion of aquatic standard dielectric and establishment of aquatic specialized genomic data platform ○ Accelerate research on aquaculture breeds by searching for useful economic and useful genes and gene markers ○ Create new industries by developing bio materials using genome big data
Project Data Type * Whole Genome sequencing,Transcriptome or Gene Expression
Sample Scope * Multiisolate

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Umbrella Project KAU200000

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Relations 내용시작

Sample Group

BioSample
Accession ID
Project Title
Sample Type
Registration Date
KASG210038 Transcriptome analysis of Platichthys stellatus Model organism or animal sample 2021-02-04
KASG210039 Omics data analysis of Platichthys stellatus Model organism or animal sample 2021-02-04

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