Detection and quantification of CBFB/MYH11 fusion transcripts in patients with inv(16)-positive acute myeloblastic leukemia by real-time RT-PCR..     ///    These data indicate that real-time RT-PCR can be used for the sensitive detection and quantification of CBFB/MYH11 transcripts in the follow-up of patients with inv(16)-positive AML..     ///     We used a newly established real-time RT-PCR assay for the quantification of the leukemia-specific CBFB/MYH11 transcripts in inv(16)-positive acute myeloblastic leukemia.     ///    "CBFB/MYH11 could be quantified over a five log range, with a detection limit of 10 molecules of a CBFB/MYH11 plasmid and a 1:10(5) dilution of RNA of the inv(16)-positive ME-1 cell line, respectively. "    ///    "Three patients relapsed, and their CBFB/MYH11 transcripts rose again to pretreatment levels. "    ///    All of these patients showed a similar decline of CBFB/MYH11 after intensive therapy.     ///    "In two patients, this increase in CBFB/MYH11 could be detected by real-time PCR before hematological relapse. "    ///    Six of these patients are in complete remission with a stable low-level or absent CBFB/MYH11 expression.     ///    "In nine patients, CBFB/MYH11 was also quantified during/after chemotherapy and autologous or allogeneic stem cell transplantation. "    ///    The expression of CBFB/MYH11 varied over a two log range without correlation to clinical response or relapse rate. 