In one case of 96 EML4-ALK fusion gene and EGFR mutation were detected.     ///    TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC..     ///    "Using variant specific real time PCR we identified EML4-ALK transcript in 17/46 (37%) specimens, using end-point PCR in 13/46 (28%) specimens and positive ALK rearrangement by FISH was detected in 8/46 (17.4%) specimens. "    ///    This assay was used to analyze the EML4-ALK gene in 96 non-selected NSCLC specimens and compared with two other methods (end-point PCR and break-apart FISH).     ///    "EML4-ALK was detected in 33/96 (34%) specimens using variant specific real time PCR, whereas in only 23/96 (24%) using end-point PCR."    ///    "In this context, an effective method for EML4-ALK detection is necessary."    ///    "EML4-ALK was detected in 33/96 (34%) specimens using variant specific real time PCR, whereas in only 23/96 (24%) using end-point PCR. "    ///    "In this context, an effective method for EML4-ALK detection is necessary. "