The EML4-ALK fusion gene status was determined with fluorescent in situ hybridization (FISH), reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemistry (IHC).    ///    "The EML4-ALK fusion gene status was determined with fluorescent in situ hybridization (FISH), reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemistry (IHC). "    ///    We attempted to clarify the feasibility of detecting the EML4-ALK fusion gene in pleural effusion cells using different methods.    ///    We attempted to clarify the feasibility of detecting the EML4-ALK fusion gene in pleural effusion cells using different methods.     ///    These results suggest that a block of pleural effusion cells can be used to detect the EML4-ALK fusion gene.     ///    "Detection of EML4-ALK in lung adenocarcinoma using pleural effusion with FISH, IHC, and RT-PCR methods."    ///    "Detection of EML4-ALK in lung adenocarcinoma using pleural effusion with FISH, IHC, and RT-PCR methods.. "    ///    RT-PCR is a good candidate method for detecting EML4-ALK in blocks of pleural effusion cells from lung cancer patients.    ///    EML4-ALK was detected in three of 66 patient samples (4.5%) with RT-PCR. 