They showed strong nuclear staining for TFE3 in immunohistochemistry, TFE3 gene rearrangement in dual-color, break-apart FISH analysis and ASPL-TFE3 type 1 fusion transcripts detected by RT-PCR and direct DNA sequencing.     ///    Establishment of an ASPL-TFE3 renal cell carcinoma cell line (S-TFE).. 