 To explore a sensitive and specific method for detection of bcl-2/IgH gene rearrangement in diffuse large B cell lymphoma (DLBCL), and verify the credibility of the established method.     ///    Fifty-two samples of pathologically diagnosed DLBCL and 10 fresh tonsil tissues were amplified using hemi-nested touch down-PCR to detect bcl-2/IgH gene rearrangement.     ///    There are false positive results using common primers for detecting bcl-2/IgH gene rearrangement.     ///    "The positive PCR products were sequenced and analyzed on the Internet, 3 of 8 cases obtained by one-way method were false positive, 5 positive cases amplified using hemi-nested PCR were all bcl-2/IgH gene rearrangement. "    ///    [Detection of bcl-2/IgH gene rearrangement in diffuse large B cell lymphoma by hemi-nested PCR]..     ///    bcl-2/IgH gene rearrangement was detected in 6 of 52 DLBCL samples and 2 of 10 fresh tonsil tissues using one-way method.     ///    bcl-2/IgH hemi-nested PCR primers were designed using the professional primer design software. 