These cells were more sensitive to crizotinib, an ALK inhibitor compared with PC-9 and H460 cells without EML4-ALK rearrangement.     ///    "In situ proximity ligation assay (PLA) originally developed for precise detection and quantification of proteins by dual recognition and amplification process was used for sensitive detection of EML4-ALK fusion oncoprotein in NSCLC cell lines (ALK negative cell: PC-9 and H460, ALK positive cell: H3122 and H2228)."    ///    Sensitive detection of EML4-ALK fusion oncoprotein of lung cancer by in situ proximity ligation assay.    ///    EML4-ALK oncogene and protein in lung cancer cells were confirmed by multiplex RT-PCR and Western blots.     ///    We detected 117 kDa variant 1 of EML4-ALK in H3122 and 90 kDa variant 3 of EML4-ALK in H2228.     ///     EML4-ALK fusion oncogene has emerged as a novel molecular target in non-small cell lung cancer (NSCLC).     ///    Highly specific and sensitive detection of EML4-ALK fusion oncoprotein is possible by in situ PLA method suggesting its clinical application..     ///    Sensitive detection of EML4-ALK fusion oncoprotein of lung cancer by in situ proximity ligation assay..     ///    "In situ proximity ligation assay (PLA) originally developed for precise detection and quantification of proteins by dual recognition and amplification process was used for sensitive detection of EML4-ALK fusion oncoprotein in NSCLC cell lines (ALK negative cell: PC-9 and H460, ALK positive cell: H3122 and H2228). "