These effects were paralleled by a decrease in the nuclear translocation of NF-?B, as demonstrated by a decrease in the expression of MyD88, degradation of IRAK-1 and I?B? expression of the nuclear NF-?B p50/p65 subunits, p-p38 MAPK and p-MEK1, and NF-?B-dependent luciferase reporter gene activity in LPS-stimulated cells.     ///    "Western blotting was used to assess the expression of the MyD88, the degradation of IRAK-1 and I?B?, the expression of the NF-?B p50/p65 subunits, the p-p38 MAPK and p-MEK1, as well as histone modifications. "