Both FISH and PCR may be useful methods to detect novel KIF5B-RET rearrangements in pulmonary adenocarcinomas rather than IHC.     ///    "53 cases of lung adenocarcinomas harbored ""triple (EGFR, KRAS and ALK)-negative"" were tested for KIF5B-RET fusions using whole-transcriptome sequencing, fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and long-range PCR. "    ///    All those three patients were also positive in FISH for both KIF5B-RET fusion and RET break-apart probes.     ///    Dual color break-apart probes and KIF5B-RET fusion probes were used for FISH.     ///    Diagnostic method for the detection of KIF5B-RET transformation in lung adenocarcinoma.    ///    Diagnostic method for the detection of KIF5B-RET transformation in lung adenocarcinoma..     ///    Primers designed for 3 different variants of KIF5B-RET fusions were used for long-range PCR.     ///    " KIF5B-RET fusions have recently been reported to occur in pulmonary adenocarcinomas, thereby being proposed as a novel genetic alteration in adenocarcinoma of the lung. "