 Echinoderm microtubule-associated proteinlike 4-anaplastic lymphoma kinase (EML4-ALK) gene fusions are detected in 3% to 13% of non-small cell lung carcinomas.     ///    "To compare reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH) methodologies for detection of EML4-ALK fusions."    ///    "To compare reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH) methodologies for detection of EML4-ALK fusions. "    ///    "EML4-ALK variant 3a/b was detectable by RT-PCR, FISH, and IHC in 4% (2 of 46) of specimens."    ///    "EML4-ALK variant 3a/b was detectable by RT-PCR, FISH, and IHC in 4% (2 of 46) of specimens. "    ///    No concordance existed among methodologies for the detection of EML4-ALK variant 1.    ///    One EML4-ALK variant 1 fusion was found to coexist with an EGFR exon 21 mutation.    ///    "Overall, the frequency of EML4-ALK variants 1 and 3a/b was 24% (11 of 46) in adenocarcinomas enriched for wild-type EGFR status. "    ///    The RT-PCR method was the most sensitive and least-subjective methodology for detection of EML4-ALK fusions..     ///    Accurate testing for detection of EML4-ALK fusions is essential for appropriate therapy selection. 